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An experiment paper of biology class in insects

Insect handling

Biology, Lund University concerning insect handling and insect allergies. Dave Young Flickr Introduction Work on insects may only be performed in rooms specially designated for it. In insects rooms were allergenic insects are handled there should be a sign posted outside saying: Work with insects present in this room. Use special protective clothing and footwear, preferably also filtering half mask and tight fitting goggles.

Introduction

Designated cleaning equipment shall be kept in this room only. Work with allergenic insects or insect specimen should be done at a ventilated workplace, such as point source ventilation, exhaust bench or fume cupboard. Use lab coat and gloves and keep them in the insect facility to avoid spreading of particles to other areas.

  • If an experiment is altered by the professor in any way, the introduction and the entire report should be about the experiment actually performed;
  • The sink should be in the same room as where the insect equipment is cleaned to avoid transportation in corridors;
  • Eyes that are red, swollen, draining or itch;
  • And, most of all, thanks to Dr;
  • Any description and explanation that is necessary for understanding the purpose of the experiment should also be included;
  • In insects rooms were allergenic insects are handled there should be a sign posted outside saying:

Put used coats in a special labelled laundry bag. Use an adhesive mat at the entrance, or special shoes in the facility. Insects to be dissected in the lab should be moved there in closed containers. To avoid unnecessary allergen proliferation avoid having too many insects in the same container.

During behavioural experiments an experiment paper of biology class in insects limit the amount of allergen in the air by reducing the number of insects at the same time or keeping them in an appropriate cage. Use lab coat and gloves also when you feed other animals with insects. For work with hymenopteras with venomous stingers wasps, bees, bumblebeessee the special paragraph below.

Cultivation and storage Cultivation and storage of insects should be done in dedicated rooms with good ventilation. Suitable material in cages for flying insects, such as butterflies, is soft walls of thin cloth or plastic. These can be easily replaced and also the spread of scales from the wings becomes lower when the butterflies fly around in this type of cage.

If you use plastic boxes with lids for butterfly culturing, these should be soaked thoroughly inside before you take off the cover for washing, etc. You can also use a spray bottle with water to to spray the waste if it releases a lot of particles into the air, and in addition put wet towel on top of the waste. The sink should be in the same room as where the insect equipment is cleaned to avoid transportation in corridors. If you work with orthopteras grasshoppers, locusts, crickets their faeces are highly allergenic, especially when dry.

Again, wet the boxes before washing to decrease the amount of dust in the room.

During waste handling and cleaning of equipment containing dust from insects, use lab coat, gloves and preferrably also filtering half mask and googles. Particle filtering half masks, also called lightweight respirators, must be of class FFP2. They come with or without vent in boxes of ten. Put up the box on the wall. Mark personal masks with your name.

Inhalation allergy risks The recommendations above concerns insects in general, some insects spread less allergenic than others. In general there are much less problems with insects which have a hard cuticle, like adult beetles, than insects with soft cuticle like butterflies or mayflies.

A risk assessment should be done before any work with insects, ie. The allergy clinic should have access to screening preparations containing butterfly scale extracts or other allergens, and can inform about various symptoms of allergy: Running or itching nose. Eyes that are red, swollen, draining or itch. Skin that itch, crack, produces blisters or blush. Allergy sufferers People who work with allergenic insects that have already developed insect allergy should; Work in ventilated workplace.

  1. Only information that is relevant to the experiment should be presented in the introduction. An enzyme is very specific in the reactions in which it undergoes.
  2. A good rule of thumb--if you don't understand a certain term or concept, you need to explain it in your introduction! In higher level biology courses the terms that are assumed to be understood do not require definition.
  3. Rather, set out to test, document, or describe. Likewise, in order to maintain its specific function, an enzyme must retain the specialized shape of its active site Campbell, 1996.

Use coat, gloves and tight fitting goggles. Masks should be stored in closed containers when not in use, to avoid contamination and be personal. Mark it with your name but do not use the same mask for a long time but change frequently.

Use separate protective clothing for cultivation and storage. Substitution of other tasks may be the only solution for people with severe insect allergy. Some insects reported to cause sensitization by inhalation: Order Example Silverfish Zygentoma — Silverfish Orthopterans Orthoptera — Crickets, grasshoppers, locusts Mayflies Ephemeroptera — Mayflies True Bugs Hemiptera — Scale insects Flies Diptera — Houseflies, fruit flies Moths and butterflies Lepidoptera — Almost all moths and butterflies Beetles Coleoptera — Cereal weevils Hymenopteras Hymenoptera — Bees, bumblebees, wasps Working with stinging instects For those who will be working with live wasps, bees, bumbleebees or other insects with venomous sting there is a special document, which should be read carefully and sign to prove you understand the instructions before starting the work.

Everyone in the Department of Biology who works with stinging insects are encouraged to use this document pdf; 160 kB.

Project-Based Learning in Insect Ecology

Hymenopteras such as bees, wasps and bumblebees have stingers, which can develop into different types of allergies if you get stung, at worst life-threatening reactions anaphylactic shock. Detailed instructions what to wear and how to behave are in the pdf document linked above.

Existing medication should be personal so that the user is familiar with the preparations purpose and expected effect. You should also do an allergy test to see if you are at high risk.

If you have developed an allergy, contact the allergy clinic for a prescription of special adrenaline injection or -spray to have available when working with wasps. Always contact a co-worker immediately if you have been stung and go to the emergency room at the hospital! Inger [dot] Ekstrom [at] biol [dot] lu [dot] se 27 June 2018.